Carnegie Mellon University                                      

   

My research interests are in lipid membrane structure and thermodynamics. Our Biophysics laboratory explores the structure of biological phospholipids using multilamellar vesicles (MLVs) and oriented lipids on substrates using X-ray diffraction with a Rigaku fixed tube source and rotating anode here at CMU, and with synchrotron radiation at the Cornell High Energy Synchrotron Source.  We also use differential scanning calorimetry to measure specific heat changes during phase transitions of aqueous dispersions of phospholipids in the form of MLVs. The third technique which yields structural information about the lipid volume as a function of temperature is density centrifugation measurements, using D₂O-H₂O mixtures.

 Recent Publications:

1.  Tristram-Nagle, S. and Nagle, J. F. 2003. Lipid Bilayers: Thermodynamics, Structure, Fluctuations and Interactions.  Chem. Phys. Lipids (Invited Review) .  (html).

2.  Petrache, H.I.,Tristram-Nagle, S., Gawrisch, K., Harries, D., Parsegian, V.A. and Nagle, J. F. 2003.^M Structure and Fluctuations of Charged Phosphatidylserine Bilayers In the Absence of Salt.  Biophys. J. (submitted).  (PDF).

3.  Eldho, N.V., Feller, S.E.,Tristram-Nagle, S., Polozov, I.V. and Gawrisch, K. 2003. Polyunsaturated Docosahexaenoic vs.Docosapentaenoic Acid- Differences in Lipid Matrix Properties from the Loss of One Double Bond.  J. Am. Chem. Soc. 125:6409-6421.  (PDF).

4.  Tristram-Nagle, S., Liu, Y., Legleiter, J. and Nagle, J. F. 2002. Structure of Gel Phase DMPC Determined by X-Ray Diffraction.  Biophys. J. 83:3324-3335.  (PDF).

5.     Lyatskaya, Y., Liu, Y., Tristram-Nagle, S. , Katsaras, J. and Nagle, J.F. 2001. Method for Obtaining Structure and Interactions from Oriented Lipid Bilayers, Phys. Rev. E 63:011907     [1-9]. (PDF)   

6.     Nagle, J.F. and Tristram-Nagle, S. 2000. Structure of Lipid Bilayers. (Invited Review for BBA). Biochim. Biophys. Acta 1469:159-195.  (PDF).  

7.     Nagle, J.F. and Tristram-Nagle, S. 2000. Lipid Bilayer Structure.  (Invited Review for Current Opinion in Structural Biology).         COSB 10:474-480 (Postscript).

8.     Katsaras, J., Tristram-Nagle, S., Liu, Y., Headrick, R.L., Fontes, E., Mason, P.C., Nagle, J.F. 2000. Clarification of the Ripple Phase of Lecithin Bilayers Using Fully Hydrated, Aligned Samples. Phys. Rev. E 61:5668-5677. (PDF)

9.     Nagle, J.F. and Tristram-Nagle, S. 1999.  Structure and Interactions of Lipid Bilayers:  Role of Fluctuations.  In Lipid Bilayers, eds. J. Katsaras,    T. Gutberlet,   Springer Verlag, N.Y.,             (pp. 1-24.)  (Postscript).

10.     Nagle, J.F., Liu, Y., Tristram-Nagle, S., Epand, R.M. and Stark, R. E.  1999.  Re-Analysis of Magic Angle Spinning NMR Determination of Intercellular Waters.  Biophys. J. 77:2062-2065. (Postscript).

11.     Tristram-Nagle, S., Isaacson, Y., Lyatskaya, Y., Liu, Y., Brummond, K., Katsaras, J. and Nagle, J.F.  1999.  Polymorphism in Myristoylpalmitoylphosphatidylcholine.  Chem. Phys. Lipids 100:101-113. (Postscript)

12.     Nagle, J.F., Petrache, H.I., Gouliaev, N., Tristram-Nagle, S., Liu, Y., Suter, R.M. and Gawrisch, K. 1998.  Multiple Mechanisms for Critical Behavior in the Biologically Relevant Phase of Lecithin Bilayers.  Phys. Rev. E. 58:7769-7776. (Postscript)

13.    Petrache, H. I., Tristram-Nagle, S. and Nagle, J. F. 1998. Fluid Phase Structure of EPC and DMPC Bilayers. Chem. Phys. Lipids 95:83-94. (Postscript)

14.  Tristram-Nagle, S., Petrache, H. I. and Nagle, J. F. 1998. Structure and Interactions of Fully Hydrated Dioleoylphosphatidylcholine Bilayers.  Biophys. J. 75:917-925.  (PDF).

15.   Petrache, H. I., Gouliaev, N., Tristram-Nagle, S., Zhang, R., Suter, R. M. and Nagle, J. F.  1998.  Interbilayer Interactions:  High Resolution X-Ray Scattering Study.  Phys. Rev. E. 57:7014-24.  (Postscript).

16.  Nagle, J. F., Tristram-Nagle, S., Takahashi, H. and Hatta, I.  1998.  Comment on DPPC Subgel Phase Formation Process.  Euro. Phys. J. B1, 399.

17.   Tristram-Nagle, S., Petrache, H. I., Suter, R. M. and Nagle, J. F. 1998. Effect of Substrate Roughness on D Spacing Supports Theoretical Resolution of Vapor Pressure Paradox. Biophys. J. 74, 1421-1427. (PDF)

18.   Tristram-Nagle, S., Moore, T., Petrache, H. and Nagle, J. F. 1998. DMSO Produces a New Subgel Phase in DPPC: DSC and X-Ray Diffraction Study.  Biochim. Biophys. Acta 1369, 19-33. (Postscript)

19.    Sun, W.,-J., Tristram-Nagle, S., Suter, R. M. and Nagle, J. F. 1996. Structure of Gel Phase Saturated Lecithin Bilayers: Temperature and Chain Length Dependence. Biophys. J. 71,  885-891.

20.  Sun, W.-J., Tristram-Nagle, S., Suter, R. M. and Nagle, J. F. 1996. Structure of the Ripple Phase in Lecithin Bilayers. Proc. Natl. Acad. Sci. U.S.A. 93, 7008-7012.

21.  Nagle, J. F., Zhang, R., Tristram-Nagle, S., Sun, W., Petrache, H. and Suter, R. M. 1996. X-Ray Structure Determination of La Phase DPPC Bilayers. Biophys. J.70, 1419-1431.

22.  Zhang, R., Tristram-Nagle, S., Sun, W., Headrick, R. L., Irving, T. C., Suter, R. M. and Nagle, J. F. 1996.  Small Angle X-Ray Scattering from Lipid Bilayers Is Well Described by Modified Caille Theory, But Not by Paracrystalline Theory.  Biophys. J. 70, 349-357.

23.  Sun, W., Tristram-Nagle, S., Suter, R. M. and Nagle, J. F. 1996. Anomalous Phase Behavior of Long Chain Saturated Lecithin Bilayers. Biochim. Biophys. Acta 1279, 17-24.

24.  Zhang, R., Sun, W., Tristram-Nagle, S., Headrick, R. L., Suter, R. M. and Nagle, J. F. 1995. Critical Fluctuations in Membranes. Phys. Rev. Lett 74, 2832-2835.

25.  Tristram-Nagle, S. and Dowd, S. 1994. X-Ray Diffraction Study of Three 19F-Labeled Dimyristoylphosphatidylcholines. J. Phys. Chem. 98, 4469-4472.

26.  Tristram-Nagle, S., Sun, W.-J., Suter, R. M. and Nagle, J. F. 1994. Kinetics of Subgel Formation in DPPC: X-Ray Diffraction Study Proves Nucleation-Growth Hypothesis. Biochim. Biophys. Acta 1191, 14-20.

27.  Tristram-Nagle, S., Zhang, R., Suter, R. M., Worthington, C. R., Sun, W.-J. and Nagle, J. F. 1993. Measurements of Chain Tilt Angle in Fully Hydrated Bilayers of Gel Phase Lecithins. Biophys. J. 64:1097-1109.

28.  Tristram-Nagle, S. and Wingert, L. M. 1990. A Thermotropic Study of  1-Deoxy-1-(N-methylocatanamido)-D-Glucitol (MEGA-8) Using Microscopy, Calorimetry and X-Ray Diffraction.  Mol. Cryst. Liq. Cryst. 188, 41-56.

29.  Tristram-Nagle, S., Wiener, M. C., Yang, C.-P. and Nagle, J. F. 1987. Kinetics of the Subtransition in Dipalmitoylphosphatidylcholine. Biochemistry 26, 4288-4294.

30.  Tristram-Nagle, S., Yang, C.-P. and Nagle, J. F. 1986. Thermodynamic Studies of Purple Membrane. Biochim. Biophys. Acta 854, 58-66.